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Mostrando publicaciones 1-7 de 7
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Biotransformation of poly(cis-1,4-isoprene) in a multiphase enzymatic reactor for continuous extraction of oligo-isoprenoid molecules
Andler-Osorio, Rodrigo; Valdés, Cristian; Díaz-Barrera, Álvaro; Steinbüchel, A. (2020)Biotechnological processes for the partial degradation or transformation of poly(cis-1,4-isoprene) rubber have been investigated during recent decades with promising results. The use of the enzyme ‘latex clearing protein’ ...
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Characterization of the latex clearing protein of the poly(cis-1,4-isoprene) and poly(trans-1,4-isoprene) degrading bacterium Nocardia nova SH22a
Vivod, Robin; Andler-Osorio, Rodrigo; Oetermann, Sylvia; Altenhoff, A. L.; Seipel, Nele; Holtkamp, Michael; Hogeback, Jens; Karst, Uwe; Steinbüchel, A. (2019)Nocardia nova SH22a is an actinobacterium capable of degrading the polyisoprenes poly(cis-1,4-isoprene) and poly(trans-1,4-isoprene). Sequencing and annotating the genome of this strain led to the identification of a single ...
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Cleavage of poly(cis-1,4-isoprene) rubber as solid substrate by cultures of Gordonia polyisoprenivorans
Andler-Osorio, Rodrigo; Hiessl, S.; Yücel, O.; Tesch, M.; Steinbüchel, A. (2018)Potential biotechnological recycling processes for rubber products include the bacterial degradation of poly(cis-1,4-isoprene) (IR) in order to achieve its total biodegradation or its biotransformation into useful products. ...
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Enhancing the synthesis of latex clearing protein by different cultivation strategies
Andler-Osorio, Rodrigo; Heger, F.; Andreeßenb, C.; Steinbüchel, A. (2019)In this study, we improved the synthesis of the latex clearing protein from Gordonia polyisoprenivorans VH2 (Lcp1VH2), a key enzyme for the initial cleavage of the rubber backbone. Cultivations using a recombinant strain ...
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Impact of additives of commercial rubber compounds on the microbial and enzymatic degradation of poly(cis-1,4-isoprene)
Altenhoff, A. L.; De Witt, Jan; Andler-Osorio, Rodrigo; Steinbüchel, A. (2019)Much fundamental research has already been performed to understand the mechanism of microbial rubber degradation. Due to the increasing amount of rubber waste, biotechnical methods to degrade that particular waste are ...
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In vitro studies on the degradation of poly(cis‐1,4‐isoprene)
Andler-Osorio, Rodrigo; Altenhoff, A. L.; Mäsing, F.; Steinbüchel, A. (2018)Cleavage of the backbone of poly(cis‐1,4‐isoprene) (IR) in solid rubber material was accomplished by the addition of partially purified latex clearing protein (Lcp1VH2) using a 200‐mL enzyme reactor. Two strategies for the ...
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Synthesis of polyhydroxyalkanoates through the biodegradation of poly(cis-1,4-isoprene) rubber
Andler-Osorio, Rodrigo; Vivod, Robin; Steinbüchel, A. (2019)The search of alternative substrates for the synthesis of polyhydroxyalkanoates (PHA) has become an important factor in order to decrease the production costs. Therefore, the use of industrial by-products or waste materials ...