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Isolation and molecular characterization of Thraustochytrium strain isolated from Antarctic peninsula and its biotechnological potential in the production of fatty acids
dc.contributor.author | Gordillo-Fuenzalida, Felipe | |
dc.contributor.author | Caamaño, Esteban | |
dc.contributor.author | Loperena, Lyliam | |
dc.contributor.author | Hinzpeter, Ivonne | |
dc.contributor.author | Pradel, Paulina | |
dc.contributor.author | Corsini, Gino | |
dc.contributor.author | Tello, Mario | |
dc.contributor.author | Lavín, Paris | |
dc.contributor.author | Gonzalez, Alex R. | |
dc.date.accessioned | 2017-11-07T19:27:10Z | |
dc.date.available | 2017-11-07T19:27:10Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | http://repositorio.ucm.cl/handle/ucm/1122 | |
dc.description.abstract | Thraustochytrids are unicellular protists belonging to the Labyrinthulomycetes class, which are characterized by the presence of a high lipid content that could replace conventional fatty acids. They show a wide geographic distribution, however their diversity in the Antarctic Region is rather scarce. The analysis based on the complete sequence of 18S rRNA gene showed that strain 34-2 belongs to the species Thraustochytrium kinnei, with 99% identity. The total lipid profile shows a wide range of saturated fatty acids with abundance of palmitic acid (16:0), showing a range of 16.1–19.7%. On the other hand, long-chain polyunsaturated fatty acids, mainly docosahexaenoic acid and eicosapentaenoic acid are present in a range of 24–48% and 6.1–9.3%, respectively. Allfactors analyzed in cells (biomass, carbon consumption and lipid content) changed with variations of culture temperature (10 ◦C and 25 ◦C). The growth in glucose at a temperature of 10 ◦C presented the most favorable conditions to produce omega-3fatty acid. This research provides the identification and characterization of a Thraustochytrids strain, with a total lipid content that presents potential applications in the production of nutritional supplements and as well biofuels. | es_CL |
dc.language.iso | en | es_CL |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | * |
dc.source | Brazilian Journal of Microbiology, 48(4), 671-679 | es_CL |
dc.subject | Thraustochytrids | es_CL |
dc.subject | Antarctic | es_CL |
dc.subject | Docosahexaenoic acid | es_CL |
dc.subject | Eicosapentaenoic acid | es_CL |
dc.subject | Temperature | es_CL |
dc.title | Isolation and molecular characterization of Thraustochytrium strain isolated from Antarctic peninsula and its biotechnological potential in the production of fatty acids | es_CL |
dc.type | Article | es_CL |
dc.ucm.facultad | Facultad de Ciencias Agrarias y Forestales | es_CL |
dc.ucm.indexacion | Scopus | es_CL |
dc.ucm.indexacion | Isi | es_CL |
dc.ucm.indexacion | Scielo | es_CL |
dc.ucm.doi | doi.org/10.1016/j.bjm.2017.01.011 | es_CL |