In vitro culture of Luma chequen from vegetative buds

Mancilla, Héctor; Quiroz, Karla; Arencibia-Rodríguez, Ariel; Carrasco, Basilio; García-González, Rolando

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dc.contributor.author	Mancilla, Héctor
dc.contributor.author	Quiroz, Karla
dc.contributor.author	Arencibia-Rodríguez, Ariel
dc.contributor.author	Carrasco, Basilio
dc.contributor.author	García-González, Rolando
dc.date.accessioned	2017-11-09T14:17:08Z
dc.date.available	2017-11-09T14:17:08Z
dc.date.issued	2013
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/1150
dc.description.abstract	Luma chequen, a small tree or large shrub belonging to the Myrtaceae family, is endemic to South America and has medicinal, nutritional and ornamental potential. However, its native habitat is deteriorating gradually, and it is suffering from the effects of fragmentation that is being caused by the conversion of forest land to agricultural land and the natural expansion of monocultural plantations of exotic species, such as Pinus radiata. The purpose of this work is to develop an effective procedure for establishing in vitro cultures of the native Chilean species L. chequen. Aseptic nodal segments were evaluated after exposure to a disinfecting agent (1% solution of sodium hypochlorite) for different lengths of time. Murashige and Skoog (MS) or Woody Plant (WPM) culture media with 6-Bencilaminopurine (BAP) or 2-isopentenil adenine (2-iP) added to a concentration of 1 mg L-1 were evaluated. Although no significant differences were observed between cultures with and ithout additives, 40.43% of the explant cultures were successfully established. Furthermore, the choice of basal medium or the addition of plant growth regulators was not found to affect the shoot formation efficiency.	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	Ciencia e Investigación Agraria, 40(3), 609-615	es_CL
dc.subject	Aseptic nodal segments	es_CL
dc.subject	In vitro culture	es_CL
dc.subject	Myrtaceae family	es_CL
dc.subject	Organogenesis	es_CL
dc.title	In vitro culture of Luma chequen from vegetative buds	es_CL
dc.type	Article	es_CL
dc.ucm.facultad	Facultad de Ciencias Agrarias y Forestales	es_CL
dc.ucm.indexacion	Scopus	es_CL
dc.ucm.indexacion	Isi	es_CL
dc.ucm.indexacion	Scielo	es_CL
dc.ucm.doi	dx.doi.org/10.4067/S0718-16202013000300013	es_CL