Genetic characterization of Japanese plum cultivars (Prunus salicina) using SSR and ISSR molecular markers

Carrasco, Basilio; Díaz, Carole; Moya, Mario; Gebauer, Marlene; García-González, Rolando

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dc.contributor.author	Carrasco, Basilio
dc.contributor.author	Díaz, Carole
dc.contributor.author	Moya, Mario
dc.contributor.author	Gebauer, Marlene
dc.contributor.author	García-González, Rolando
dc.date.accessioned	2017-11-16T14:08:30Z
dc.date.available	2017-11-16T14:08:30Z
dc.date.issued	2012
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/1258
dc.description.abstract	The genetic characterization of 29 elite Japanese plum cultivars (Prunus salicina) and 4 Prunus cultivars was carried out by analyzing 97 Simple Sequence Repeat (SSR) alleles and 232 binary Inter Simple Sequence Repeat (ISSR) loci. A high level of genetic variability was found for these two molecular markers among the Japanese plum cultivars compared to other Prunus species. On average, the variability found by analyzing the SSR alleles were Na = 12.1, Ne = 5.2, Ho = 0.9, He = 0.8 and F= -0.127, whereas ISSR yielded values of h = 0.15 and I = 0.27. The genetic relationship among cultivars was estimated with Principal Coordinate Analysis (PCA) and a Bayesian clustering approach using the software program Structure. This program identified two subgroups (k=2). The first group included cultivars of four Prunus species: P. salicina, P. armeniaca, P. domestica and P. ceracifera, whose memberships ranged between 0.74 and 1.0. The second group included 19 Japanese plum cultivars and one plumcot cultivar, with memberships between 0.57 and 0.99. With some exceptions, similar relationships among cultivars were foundPCA. The level of genetic differentiation between two groups was low (Gst =0.055 and ST =0.04), and a low level of linkage disequilibrium (LD) was observed for all allele combinations. These results suggest that the high level of genetic variability, the low level of LD and the scarce degree of differentiation detected by Structure between the two genetic groups can be explained by the self-incompatibility mechanism that favors the exchange between genetically distant Prunus cultivars and by the intra- and interspecific hybridization strategies frequently used in plum breeding programs.	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	Ciencia e Investigación Agraria, 39(3), 533-543	es_CL
dc.subject	AMOVA	es_CL
dc.subject	Bayesian analysis	es_CL
dc.subject	Genetic distance	es_CL
dc.subject	PCA	es_CL
dc.subject	Plum	es_CL
dc.subject	Structure	es_CL
dc.title	Genetic characterization of Japanese plum cultivars (Prunus salicina) using SSR and ISSR molecular markers	es_CL
dc.type	Article	es_CL
dc.ucm.facultad	Facultad de Ciencias Agrarias y Forestales	es_CL
dc.ucm.indexacion	Scopus	es_CL
dc.ucm.indexacion	Isi	es_CL
dc.ucm.indexacion	Scielo	es_CL
dc.ucm.doi	dx.doi.org/10.4067/S0718-16202012000300012	es_CL