Inter simple sequence repeat markers associated with flowering time duration in the chilean strawberry (Fragaria chiloensis)

Carrasco, Basilio; Retamales, Jorge B.; Quiroz, Karla; Garriga, Miguel; Caligari, Peter D. S.; García-González, Rolando

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dc.contributor.author	Carrasco, Basilio
dc.contributor.author	Retamales, Jorge B.
dc.contributor.author	Quiroz, Karla
dc.contributor.author	Garriga, Miguel
dc.contributor.author	Caligari, Peter D. S.
dc.contributor.author	García-González, Rolando
dc.date.accessioned	2017-11-16T19:57:07Z
dc.date.available	2017-11-16T19:57:07Z
dc.date.issued	2013
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/1263
dc.description.abstract	The flowering and fructification period of the Chilean strawberry (Fragaria chiloensis (L.) Duch.) is restricted to approximately 2 mo, which seriously limits the commercial development of the species. The objective of the current investigation was to identify Inter Simple Sequence Repeat (ISSR) polymorphisms associated with flowering duration in accessions of F. chiloensis. The flowering duration data related to 41 accessions obtained over 3 years were analyzed, and a set of 40 ISSR primers tested. Two clusters were obtained through the Partitioning Around Medoids algorithm, with 23 vs. 18 accessions, and 64.1 vs. 95.6 days of flowering, respectively. Flowering duration, between the two groups, was significantly different. The years also revealed a significant effect, on flowering duration, between the two groups. Ten of the ISSR primers tested revealed reproducible and consistent banding patterns, displaying a total of 106 putative loci, of which 79 were polymorphic. Three ISSR loci (811779, 844670, 841980) were identified owing to their significant contribution to the differentiation among the accessions. Similarly, three ISSR loci (811600, 8121180, 841980) exhibited a significant correlation with the flowering duration variation. Locus 841980, which presented the highest level of correlation with flowering duration, was isolated, cloned and sequenced, but it showed only a low level of homology with the relevant sequences published in the GenBank database. The identified loci showing high correlation with the flowering time could help build Quantitative Trait Loci (QTL) maps for selection and improvement programs in the Fragaria sp. genus or other related species.	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	Journal of Agricultural Science and Technology, 15(6), 1195-1207	es_CL
dc.subject	ISSR polymorphisms	es_CL
dc.subject	Molecular markers	es_CL
dc.subject	Association analysis	es_CL
dc.title	Inter simple sequence repeat markers associated with flowering time duration in the chilean strawberry (Fragaria chiloensis)	es_CL
dc.type	Article	es_CL
dc.ucm.facultad	Facultad de Ciencias Agrarias y Forestales	es_CL
dc.ucm.indexacion	Scopus	es_CL
dc.ucm.indexacion	Isi	es_CL