Patrón EPIYA en cepas de Helicobacter pylori CagA positivas en pacientes del Hospital Regional de Talca

González-Bonet, Ileana; Romero-Elias, María; Morales-Mejías, Erik; Valdez-Moyano, Eliana; Cofré-Loyola, Cecilia; Manques-Maldonado, Belarmino; Rojas, Armando

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dc.contributor.author	González-Bonet, Ileana
dc.contributor.author	Romero-Elias, María
dc.contributor.author	Morales-Mejías, Erik
dc.contributor.author	Valdez-Moyano, Eliana
dc.contributor.author	Cofré-Loyola, Cecilia
dc.contributor.author	Manques-Maldonado, Belarmino
dc.contributor.author	Rojas, Armando
dc.date.accessioned	2017-11-21T18:57:28Z
dc.date.available	2017-11-21T18:57:28Z
dc.date.issued	2017
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/1294
dc.description.abstract	Background: The clinical outcome of Helicobacter pylori (H. pylori) infection has been related to the presence of CagA protein. This protein is highly polymorphic and its oncogenic ability depends on the number and type of tyrosine phosphorylation sites in the EPIYAs repeat sequences (A, B, C and D). Aim: To determine the EPIYA patterns of the CagA gene in H. pylori strains and its relationship with gastrointestinal pathology in infected patients of the Regional Hospital of Talca. Material and Methods: The strains were isolated from gastric biopsies and characterized by bacteriological and molecular methods. Gastrointestinal pathology was characterized by histopathological analysis. For the determination of the presence of the cagA gene and the EPIYAs standards, the conventional PCR technique was used. Results: 138 DNA samples from H. pylori strains were analyzed. 92.0% (127/138) of the isolates carried the cagA gene, of which 66 (52.0%) corresponded to the EPIYA-ABC pattern, 43 (33.8%) to the EPIYA-ABCC pattern and 21 16.5%) to the EPIYA-ABCCC phosphorylation pattern. 50.4% (64/127) of cagA positive strains isolated from dyspeptic patients in the Maule region have more than two C sites of phosphorylation. The number of EPIYAs C motifs was associated with the presence of more severe histopathological damage in the gastric mucosa.	es_CL
dc.language.iso	es	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	Revista Médica del Maule, 33(1), 8-13	es_CL
dc.subject	Helicobacter pylori	es_CL
dc.subject	EPIYA motifs	es_CL
dc.subject	CagA protein	es_CL
dc.title	Patrón EPIYA en cepas de Helicobacter pylori CagA positivas en pacientes del Hospital Regional de Talca	es_CL
dc.title.alternative	EPIYA pattern in Helicobacter pylori CagA positive strains in patients of the Regional Hospital of Talca	es_CL
dc.type	Article	es_CL
dc.ucm.facultad	Facultad de Medicina	es_CL