Molecular tools for rapid and accurate detection of black truff (Tuber melanosporum Vitt.) in inoculated nursery plants and commercial plantations in Chile

Cordero, Cecilia; Cáceres-Ruz, Pablo; González-Silva, Gloria; Quiroz, Karla; Bravo, Carmen; Ramírez, Ricardo; Caligari, Peter D. S.; Carrasco, Basilio; García-González, Rolando

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dc.contributor.author	Cordero, Cecilia
dc.contributor.author	Cáceres-Ruz, Pablo
dc.contributor.author	González-Silva, Gloria
dc.contributor.author	Quiroz, Karla
dc.contributor.author	Bravo, Carmen
dc.contributor.author	Ramírez, Ricardo
dc.contributor.author	Caligari, Peter D. S.
dc.contributor.author	Carrasco, Basilio
dc.contributor.author	García-González, Rolando
dc.date.accessioned	2017-11-30T19:24:05Z
dc.date.available	2017-11-30T19:24:05Z
dc.date.issued	2011
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/1514
dc.description.abstract	Truffle (Tuber melanosporum Vitt.) culture is an agroforestry sector in Chile of increasing interest due to the high prices that truffles fetch in the national market and the recent evidence that its commercial production is possible in Chilean climatic and soil conditions. In this study, the efficiency of three methods of DNA extraction from a mix of 5 g of soil and roots from both nursery and field plants of Quercus ilex L. mycorrhized with T. melanosporum were evaluated, and a simple and reproducible protocol was established. Detection of T. melanosporum was performed by the technique of cleaved amplified polymorphic sequence (CAPS) from amplicons generated with the primers ADL1 (5´-GTAACGATAAAGGCCATCTATAGG-3´) and ADL3 (5´-CGTTTTTCCTGAACTCTTCATCAC-3`), where a restriction fragment of 160 bp specific for T. melanosporum was generated, which allows the discrimination of this species from the rest of the species belonging to the Tuber sp. genus. Direct detection of T. melanosporum in one step was also obtained by polymerase chain reaction (PCR) from total DNA isolated from mycorrhized roots and with the primers ITSML (5´-TGGCCATGTGTCAGATTTAGTA-3´) and ITSLNG (5´-TGATATGCTTAAGTTCAGCGGG-3´), generating a single amplicon of 440 bp. The molecular detection of T. melanosporum by the methods presented here will allow the rapid and accurate detection of mycorrhization of trees, both under nursery and field conditions. This technology will also provide more security to farmers by controlling the quality of the mycorrhized trees they will plant and also by following the mycorrhization status of established orchards.	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	Chilean Journal of Agricultural Research, 71(3), 488-494	es_CL
dc.subject	Tuber melanosporum	es_CL
dc.subject	Ectomycorrhizas	es_CL
dc.subject	Molecular markers	es_CL
dc.title	Molecular tools for rapid and accurate detection of black truff (Tuber melanosporum Vitt.) in inoculated nursery plants and commercial plantations in Chile	es_CL
dc.type	Article	es_CL
dc.ucm.facultad	Facultad de Ciencias Agrarias y Forestales	es_CL
dc.ucm.indexacion	Scopus	es_CL
dc.ucm.indexacion	Isi	es_CL
dc.ucm.indexacion	Scielo	es_CL
dc.ucm.doi	dx.doi.org/10.4067/S0718-58392011000300022	es_CL