In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae)

Quiroz, Karla; Saavedra, Jessica; Vogel, Hermine; Verdugo, Gabriela; Caligari, Peter D. S.; García-González, Rolando

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dc.contributor.author	Quiroz, Karla
dc.contributor.author	Saavedra, Jessica
dc.contributor.author	Vogel, Hermine
dc.contributor.author	Verdugo, Gabriela
dc.contributor.author	Caligari, Peter D. S.
dc.contributor.author	García-González, Rolando
dc.date.accessioned	2017-09-12T19:34:40Z
dc.date.available	2017-09-12T19:34:40Z
dc.date.issued	2017
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/155
dc.description.abstract	Premise of the study: Chloraea crispa is a terrestrial Orchidaceae species native to Chile, characterized by a beautiful and showy inflorescence. The species has a great potential for commercial exploitation in the cut flower industry, but it is essential to improve propagation methods to avoid endangering its natural populations. Because this species is hard to propagate using traditional greenhouse techniques, in vitro techniques offer an effective tool for its large-scale production in terms of germination, growth, and propagation. Methods: The current study evaluated the effect of the culture medium on the asymbiotic germination of C. crispa seeds, as well as the effects of the plant growth regulators 6-benzylaminopurine and indole-3-butyric acid. Different light regimes were also studied. Results: A significant effect was observed for the interaction between culture media and light regime on the morphogenic response of the seeds. The highest rate of embryonic germination was obtained in Van Waes medium supplemented with 0.1 mg·L of 6-benzylaminopurine. Discussion: For the first time, asymbiotic culture of this species using biotechnology tools has been developed. Plantlets developed very well under in vitro conditions, allowing the possibility to propagate and store genetic material for conservation and domestication purposes.	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	Applications in Plant Sciences, 5(8), 1600142	es_CL
dc.subject	Asymbiotic in vitro germination	es_CL
dc.subject	Chloraea crispa	es_CL
dc.subject	Orchidaceae	es_CL
dc.subject	Tissue culture	es_CL
dc.title	In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae)	es_CL
dc.type	Article	es_CL
dc.ucm.facultad	Facultad de Ciencias Agrarias y Forestales	es_CL
dc.ucm.indexacion	Scopus	es_CL
dc.ucm.indexacion	Isi	es_CL
dc.ucm.doi	doi.org/10.3732/apps.1600142	es_CL