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Single-cell sequencing unveils the lifestyle and CRISPR-basedpopulation history of Hydrotalea sp. in acid mine drainage

dc.contributor.authorMedeiros, Julliane D.
dc.contributor.authorLeite, Laura R.
dc.contributor.authorPylro, V. S.
dc.contributor.authorOliveira, F. S.
dc.contributor.authorAlmeida, V. M.
dc.contributor.authorFernandes, G. R.
dc.contributor.authorSalim, A C. M.
dc.contributor.authorAraújo, F. M. G
dc.contributor.authorVolpini, A. C.
dc.contributor.authorOliveira, G.
dc.contributor.authorCuadros-Orellana, Sara
dc.date.accessioned2018-01-04T12:21:03Z
dc.date.available2018-01-04T12:21:03Z
dc.date.issued2017
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/1658
dc.description.abstractAcid mine drainage (AMD) is characterized by an acid and metal-rich run-off thatoriginates from mining systems. Despite having been studied for many decades,much remains unknown about the microbial community dynamics in AMD sites,especially during their early development, when the acidity is moderate. Here, wedescribe draft genome assemblies from single cells retrieved from an early-stageAMD sample. These cells belong to the genus Hydrotalea and are closely related toHydrotalea flava. The phylogeny and average nucleotide identity analysis suggestthat all single amplified genomes (SAGs) form two clades that may represent differ-ent strains. These cells have the genomic potential for denitrification, copper andother metal resistance. Two coexisting CRISPR-Cas loci were recovered acrossSAGs, and we observed heterogeneity in the population with regard to the spacersequences, together with the loss of trailer-end spacers. Our results suggest thatthe genomes of Hydrotalea sp. strains studied here are adjusting to a quickly chang-ing selective pressure at the microhabitat scale, and an important form of this selec-tive pressure is infection by foreign DNA.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceMolecular Ecology, 26(20), 5541–5551es_CL
dc.subjectAcid mine drainagees_CL
dc.subjectCRISPR-Cas systemses_CL
dc.subjectHydrotalea sp.es_CL
dc.subjectMetal resistancees_CL
dc.subjectSingle-cell genomicses_CL
dc.titleSingle-cell sequencing unveils the lifestyle and CRISPR-basedpopulation history of Hydrotalea sp. in acid mine drainagees_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias Agrarias y Forestaleses_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.urisibib2.ucm.cl:2048/login?url=https://onlinelibrary.wiley.com/doi/abs/10.1111/mec.14294es_CL
dc.ucm.doidoi.org/10.1111/mec.14294es_CL


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