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Reduction of high-fat diet-induced liver proinflammatory state by eicosapentaenoic acid plus hydroxytyrosol supplementation: involvement of resolvins RvE1/2 and RvD1/2

dc.contributor.authorEcheverría, Francisca
dc.contributor.authorValenzuela, Rodrigo
dc.contributor.authorEspinosa, Alejandra
dc.contributor.authorBustamante, Andrés
dc.contributor.authorÁlvarez, Daniela
dc.contributor.authorGonzalez-Mañan, Daniel
dc.contributor.authorOrtiz, Macarena
dc.contributor.authorSoto-Alarcon, Sandra
dc.contributor.authorVidela, Luis A.
dc.date.accessioned2023-03-15T12:50:13Z
dc.date.available2023-03-15T12:50:13Z
dc.date.issued2019
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/4526
dc.description.abstractHigh-fat diet (HFD)-fed mice show obesity with development of liver steatosis and a proinflammatory state without establishing an inflammatory reaction. The aim of this work was to assess the hypothesis that eicosapentaenoic acid (EPA) plus hydroxytyrosol (HT) supplementation prevents the inflammatory reaction through enhancement in the hepatic resolvin content in HFD-fed mice. Male C57BL/6J mice were fed an HFD or a control diet and supplemented with EPA (50 mg/kg/day) and HT (5 mg/kg/day) or their respective vehicles for 12 weeks. Measurements include liver levels of EPA, DHA and palmitate (gas chromatography), liver resolvins and triglyceride (TG) and serum aspartate transaminase (AST) (specific kits) and hepatic and serum inflammatory markers (quantitative polymerase chain reaction and enzyme-linked immunosorbent assay). Compared to CD, HFD induced body weight gain, liver steatosis and TG accumulation, with up-regulation of proinflammatory markers in the absence of histological inflammation or serum AST changes; these results were accompanied by higher hepatic levels of resolvins RvE1, RvE2, RvD1 and RvD2, with decreases in EPA and DHA contents. EPA+HT supplementation in HFD feeding synergistically reduced the steatosis score over individual treatments and increased the hepatic levels of EPA, DHA and resolvins, with attenuation of proinflammatory markers. Lack of progression of HFD-induced proinflammatory state into overt inflammation is associated with resolvin up-regulation, which is further increased by EPA+HT supplementation eliciting steatosis attenuation. These findings point to the importance of combined protocols in hepatoprotection due to the involvement of cross-talk mechanisms, which increase effectiveness and diminish dosages, avoiding undesirable effects.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceThe Journal of Nutritional Biochemistry, 63, 35-43es_CL
dc.subjectHigh-fat dietes_CL
dc.subjectLiver steatosises_CL
dc.subjectHepatic proinflammatory statuses_CL
dc.subjectEPAes_CL
dc.subjectHydroxytyrosoles_CL
dc.subjectResolvinses_CL
dc.titleReduction of high-fat diet-induced liver proinflammatory state by eicosapentaenoic acid plus hydroxytyrosol supplementation: involvement of resolvins RvE1/2 and RvD1/2es_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias de la Saludes_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.urisibib2.ucm.cl:2048/login?url=https://www.sciencedirect.com/science/article/pii/S095528631830603Xes_CL
dc.ucm.doidoi.org/10.1016/j.jnutbio.2018.09.012es_CL


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Atribución-NoComercial-SinDerivadas 3.0 Chile
Excepto si se señala otra cosa, la licencia de la publicación se describe como Atribución-NoComercial-SinDerivadas 3.0 Chile