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Maquiberry cystatins: recombinant expression, characterization, and use to protect tooth dentin and enamel

dc.contributor.authorPereira de Souza, Eduardo
dc.contributor.authorFerro, Milene
dc.contributor.authorTaioqui Pelá, Vinicius
dc.contributor.authorFernanda-Carlos, Thais
dc.contributor.authorGiannico Borges, Cecília Guimarães
dc.contributor.authorAkemi Taira, Even
dc.contributor.authorOliveira Ventura, Talita Mendes
dc.contributor.authorArencibia, Ariel Domingo
dc.contributor.authorRabelo Buzalaf, Marília Afonso
dc.contributor.authorHenrique-Silva, Flavio
dc.date.accessioned2023-07-10T19:52:46Z
dc.date.available2023-07-10T19:52:46Z
dc.date.issued2023
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/4860
dc.description.abstractPhytocystatins are proteinaceous competitive inhibitors of cysteine peptidases involved in physiological and defensive roles in plants. Their application as potential therapeutics for human disorders has been suggested, and the hunt for novel cystatin variants in different plants, such as maqui (Aristotelia chilensis), is pertinent. Being an understudied species, the biotechnological potential of maqui proteins is little understood. In the present study, we constructed a transcriptome of maqui plantlets using next-generation sequencing, in which we found six cystatin sequences. Five of them were cloned and recombinantly expressed. Inhibition assays were performed against papain and human cathepsins B and L. Maquicystatins can inhibit the proteases in nanomolar order, except MaquiCPIs 4 and 5, which inhibit cathepsin B in micromolar order. This suggests maquicystatins’ potential use for treating human diseases. In addition, since we previously demonstrated the efficacy of a sugarcane-derived cystatin to protect dental enamel, we tested the ability of MaquiCPI-3 to protect both dentin and enamel. Both were protected by this protein (by One-way ANOVA and Tukey’s Multiple Comparisons Test, p < 0.05), suggesting its potential usage in dental products.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceBiomedicines, 11(5), 1360es_CL
dc.subjectAristotelia chilensises_CL
dc.subjectPhytocystatines_CL
dc.subjectCysteine protease inhibitores_CL
dc.subjectCathepsines_CL
dc.subjectCysteine proteasees_CL
dc.subjectDentistryes_CL
dc.subjectDental erosiones_CL
dc.titleMaquiberry cystatins: recombinant expression, characterization, and use to protect tooth dentin and enameles_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias Agrarias y Forestaleses_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.urimdpi.com/2227-9059/11/5/1360es_CL
dc.ucm.doidoi.org/10.3390/biomedicines11051360es_CL


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