Mostrar el registro sencillo de la publicación
Dual effect of serotonin on the dendritic growth of cultured hippocampal neurons: Involvement of 5-HT1A and 5-HT7 receptors
dc.contributor.author | Aliaga-Rojas, Esteban | |
dc.contributor.author | Rojas, P.S. | |
dc.contributor.author | Aguayo, Felipe I. | |
dc.contributor.author | Neira, D. | |
dc.contributor.author | Muñoz, J.P. | |
dc.contributor.author | Tejos-Bravo, Macarena | |
dc.contributor.author | Parra, Claudio S. | |
dc.contributor.author | Fiedler, Jenny L. | |
dc.date.accessioned | 2017-10-30T15:20:49Z | |
dc.date.available | 2017-10-30T15:20:49Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | http://repositorio.ucm.cl/handle/ucm/944 | |
dc.description.abstract | Serotonin acts through its receptors (5-HTRs) to shape brain networks during development and modulates essential functions in mature brain. The 5-HT1AR is mainly located at soma of hippocampal neurons early during brain development and its expression gradually shifts to dendrites during postnatal development. The 5-HT7R expressed early during hippocampus development, shows a progressive reduction in its expression postnatally. Considering these changes during development, we evaluated in cultured hippocampal neurons whether the 5- HT1AR and 5-HT7R change their expression, modulate dendritic growth, and activate signaling pathways such as ERK1/2, AKT/GSK3β and LIMK/cofilin, which may sustain dendrite outgrowth by controlling cytoskeleton dynamics. We show that mRNA levels of both receptors increase between 2 and 7 DIV; however only protein levels of 5- HT7R increase significantly at 7 DIV. The 5-HT1AR is preferentially distributed in the soma, while 5-HT7R displays a somato-dendritic localization at 7 DIV. Through stimulation with 5-HT at 7 DIV during 24 h and using specific antagonists, we determined that 5-HT1AR decreases the number of primary and secondary dendrites and restricts the growth of primary dendrites. The activation of 5-HT1AR and 5-HT7R promotes the growth of short secondary dendrites and triggers ERK1/2 and AKT phosphorylation through MEK and PI3K activation respectively; without changes in the phosphorylation of LIMK and cofilin. We conclude that 5-HT1AR restricts dendritogenesis and outgrowth of primary dendrites, but that both 5-HT1AR and 5-HT7R promote secondary dendrite outgrowth. These data support the role of 5-HT in neuronal outgrowth during development and provide insight into cellular basis of neurodevelopmental disorders. | es_CL |
dc.language.iso | en | es_CL |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | * |
dc.source | Molecular and Cellular Neuroscience, 85, 148–161 | es_CL |
dc.subject | Serotonin | es_CL |
dc.subject | 5-HT1AR | es_CL |
dc.subject | 5-HT7R | es_CL |
dc.subject | Outgrowth | es_CL |
dc.subject | Dendrite | es_CL |
dc.subject | Hippocampal neurons | es_CL |
dc.subject | ERK1/2 | es_CL |
dc.subject | AKT | es_CL |
dc.subject | GSK3β | es_CL |
dc.subject | LIMK | es_CL |
dc.subject | Cofilin | es_CL |
dc.title | Dual effect of serotonin on the dendritic growth of cultured hippocampal neurons: Involvement of 5-HT1A and 5-HT7 receptors | es_CL |
dc.type | Article | es_CL |
dc.ucm.facultad | Facultad de Ciencias de la Salud | es_CL |
dc.ucm.indexacion | Scopus | es_CL |
dc.ucm.indexacion | Isi | es_CL |
dc.ucm.uri | sibib2.ucm.cl:2048/login?url=https://www.sciencedirect.com/science/article/pii/S104474311730180X?via%3Dihub | es_CL |
dc.ucm.doi | doi.org/10.1016/j.mcn.2017.09.009 | es_CL |
Ficheros en la publicación
Ficheros | Tamaño | Formato | Ver |
---|---|---|---|
No hay ficheros asociados a esta publicación. |