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An approach for micropropagation of blueberry (Vaccinium corymbosum L.) plants mediated by temporary immersion bioreactors (TIBs)
Arencibia-Rodríguez, Ariel; Vergara, Carolina; Quiroz, Karla ; Carrasco, Basilio; Bravo, Carmen; García-González, Rolando (2013)A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue ...
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Application of inter-simple sequence repeats relative to simple sequence repeats as a molecular marker system for indexing blueberry cultivars
Garriga, Miguel; Parra, Pablo A.; Caligari, Peter D. S.; Retamales, Jorge B.; Carrasco, Basilio; Lobos, Gustavo A.; García-González, Rolando (2013)Chile, the major exporter of blueberries in South America, grows two species commercially, the highbush blueberry (Vaccinium corymbosum L.) and the rabbiteye blueberry (Vaccinium ashei Reade). Considering the increasing ...
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Breeding in peach, cherry and plum: From a tissue culture, genetic, transcriptomic and genomic perspective
Carrasco, Basilio; Meisel, Lee; Gebauer, Marlene; García-González, Rolando; Silva, Herman (2013)This review is an overview of traditional and modern breeding methodologies being used to develop new Prunus cultivars (stone fruits) with major emphasis on peach, sweet cherry and Japanese plum. To this end, common ...
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Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)
García-González, Rolando; Pico Mendoza, José; Quiroz, Karla ; Carrasco, Basilio; Cáceres-Ruz, Pablo; Chong-Pérez, Borys; Pino, Hugo; Sieltgens, Marjorie; Greck, Eglis; Caligari, Peter D. S. (2018)Background: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae ...
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Efficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile
Cáceres-Ruz, Pablo; Cordero, Cecilia; González-Silva, Gloria; Quiroz, Karla ; Bobadilla, Juan; Bravo-Castillo, Carmen; Caligari, Peter D. S.; Carrasco, Basilio; García-González, Rolando (2012)A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ...
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Establishment of photomixotrophic cultures for raspberry micropropagation in temporary immersion bioreactors (TIBs)
Arencibia-Rodríguez, Ariel; Vergara, Carolina; Quiroz, Karla ; Carrasco, Basilio; García-González, Rolando (2013)A novel protocol for raspberry (Rubus spp.) micropropagation in photomixotrophic conditions was optimized for the commercial genotypes Heritage, Meeker and Amity. Plant cultures were established in Temporary Immersion ...
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Genetic and morphological characterization of the endangered Austral papaya Vasconcellea chilensis (Planch. ex A. DC.) Solms
Carrasco, Basilio; García-González, Rolando; Díaz, C.; Ávila, P.; Cáceres-Ruz, Pablo; Lobos, Gustavo A.; Silva, H.; Caligari, Peter D. S. (2014)The Austral papaya (Vasconcellea chilensis) is an endangered species that has valuable characteristics for introgression into other papaya species. These characteristics include disease resistance, cold tolerance and latex ...
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Genetic characterization of Japanese plum cultivars (Prunus salicina) using SSR and ISSR molecular markers
Carrasco, Basilio; Díaz, Carole; Moya, Mario; Gebauer, Marlene; García-González, Rolando (2012)The genetic characterization of 29 elite Japanese plum cultivars (Prunus salicina) and 4 Prunus cultivars was carried out by analyzing 97 Simple Sequence Repeat (SSR) alleles and 232 binary Inter Simple Sequence Repeat ...
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Genetic diversity and population structure of chilean blueberry Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae)
Pico Mendoza, José; García-González, Rolando; Quiroz, Karla ; Pinoargote, Miryan; Rodríguez-Alvarez, Yohaily; Chong-Pérez, Borys; Cáceres-Ruz, Pablo; Pino, Hugo; Caligari, Peter D. S.; Carrasco, Basilio (2020)Gaultheria pumila (L.f.) D.J. Middleton is a native shrub of Chile that produces edible berry fruits. This species is related to the cultivated Vaccinium species; for this reason it is currently called Chilean blueberry ...
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Genetic diversity and population structure of Chilean blueberry Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae)
Pico Mendoza, José; Garcia-Gonzáles, Rolando; Quiroz, Karla ; Pinoargote, Miryan; Rodríguez-Alvarez, Yohaily; Chong-Pérez, Borys; Cáceres-Ruz, Pablo; Pino, Hugo; Caligari, Peter D. S.; Carrasco, Basilio (2020)Gaultheria pumila (L.f.) D.J. Middleton is a native shrub of Chile that produces edible berry fruits. This species is related to the cultivated Vaccinium species; for this reason it is currently called Chilean blueberry ...
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Germination capacity of Annona deceptrix (Westra) H. Rainer (Annonaceae) an endemic and endangered species in Manabí, Ecuador
Pico Mendoza, José; Pinoargote, Miryan; Madrid, Luis; Flor, Juan; Álava, Janner; Sancán, Gema; Carrasco, Basilio; Limongi, Ricardo; Peña, Geover; Quiroz, Karla (2020)Annona deceptrix is an endemic and endangered tree from Ecuador according to the IUCN Red List of Endangered Species™. Its endangered status has been related with anthropogenic activities and some intrinsic characteristics ...
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Heterogeneous genetic structure in a natural population of Raulí (Nothofagus nervosa)
Carrasco, Basilio; Eaton, Lafayette; Letelier, Luis; Díaz, Carole; García-González, Rolando (2011)Es común encontrar deficiencias de heterocigotos en poblaciones naturales de especies forestales alógamas, lo cual se explicaría por un aumento del nivel de consanguinidad. Se ha postulado que la consanguinidad es causada ...
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In vitro culture of Luma chequen from vegetative buds
Mancilla, Héctor; Quiroz, Karla ; Arencibia-Rodríguez, Ariel; Carrasco, Basilio; García-González, Rolando (2013)Luma chequen, a small tree or large shrub belonging to the Myrtaceae family, is endemic to South America and has medicinal, nutritional and ornamental potential. However, its native habitat is deteriorating gradually, and ...
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In vitro Propagation of Cedar (Cedrela odorata L.) from juvenile shoots
García-González, Rolando; Delgado, Miladys; González, Yailín; González, Aníbal; Garriga, Miguel; Caligari, Peter D. S.; Carrasco, Basilio; Quiroz, Karla (2011)Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to ...
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In vitro propagation of gaultheria pumila (L.f.) D.J. Middleton (Ericaceae), a chilean native berry with commercial potential
Pico Mendoza, José; Garcia-Gonzáles, Rolando; Quiroz, Karla ; Chong-Pérez, Borys; Pino, Hugo; Carrasco, Basilio (2021)A micropropagation protocol for G. pumila was developed. Young shoots were collected during the growing season (October to December 2016) from a wild population in the Villarrica Volcano area in the Araucanía Region of ...
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Inter simple sequence repeat markers associated with flowering time duration in the chilean strawberry (Fragaria chiloensis)
Carrasco, Basilio; Retamales, Jorge B.; Quiroz, Karla ; Garriga, Miguel; Caligari, Peter D. S.; García-González, Rolando (2013)The flowering and fructification period of the Chilean strawberry (Fragaria chiloensis (L.) Duch.) is restricted to approximately 2 mo, which seriously limits the commercial development of the species. The objective of the ...
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Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)
Quiroz, Karla ; Berríos- Muñoz, Miguel; Carrasco, Basilio; Retamales, Jorge B.; Caligari, Peter D. S.; García-González, Rolando (2017)Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propaga‑ tion, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro ...
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Molecular tools for rapid and accurate detection of black truff (Tuber melanosporum Vitt.) in inoculated nursery plants and commercial plantations in Chile
Cordero, Cecilia; Cáceres-Ruz, Pablo; González-Silva, Gloria; Quiroz, Karla ; Bravo, Carmen; Ramírez, Ricardo; Caligari, Peter D. S.; Carrasco, Basilio; García-González, Rolando (2011)Truffle (Tuber melanosporum Vitt.) culture is an agroforestry sector in Chile of increasing interest due to the high prices that truffles fetch in the national market and the recent evidence that its commercial production ...
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Morphometric and phytochemical characterization of chaura fruits (Gaultheria pumila): A native Chilean berry with commercial potential
Villagra-Quero, Evelyn; Campos-Hernández, Carola; Cáceres-Ruz, Pablo; Cabrera, Gustavo; Bernardo, Yamilé; Carrasco, Basilio; Caligari, Peter D. S.; Pico, José; García-González, Rolando; Arencibia-Rodríguez, Ariel (2014)Background: For the first time, a morphometric characterization of chaura (Gaultheria pumila) fruits has been conducted between natural populations growing in the Villarrica National Park, Araucania Region, Chile. Chaura ...