An approach for micropropagation of blueberry (Vaccinium corymbosum L.) plants mediated by temporary immersion bioreactors (TIBs)

Arencibia-Rodríguez, Ariel; Vergara, Carolina; Quiroz, Karla; Carrasco, Basilio; Bravo, Carmen; García-González, Rolando

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dc.contributor.author	Arencibia-Rodríguez, Ariel
dc.contributor.author	Vergara, Carolina
dc.contributor.author	Quiroz, Karla
dc.contributor.author	Carrasco, Basilio
dc.contributor.author	Bravo, Carmen
dc.contributor.author	García-González, Rolando
dc.date.accessioned	2017-11-13T19:28:04Z
dc.date.available	2017-11-13T19:28:04Z
dc.date.issued	2013
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/1230
dc.description.abstract	A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced medium, and light intensity of 60 M m−2 ·s−1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	American Journal of Plant Sciences, 4, 1022-1028	es_CL
dc.subject	Temporary immersion bioreactors	es_CL
dc.subject	Vaccinium corymbosum L.	es_CL
dc.subject	Photomixotrophic	es_CL
dc.subject	Blueberry	es_CL
dc.subject	Micropropagation	es_CL
dc.title	An approach for micropropagation of blueberry (Vaccinium corymbosum L.) plants mediated by temporary immersion bioreactors (TIBs)	es_CL
dc.type	Article	es_CL
dc.ucm.facultad	Facultad de Ciencias Agrarias y Forestales	es_CL
dc.ucm.doi	dx.doi.org/10.4236/ajps.2013.45126	es_CL