Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)

Quiroz, Karla; Berríos- Muñoz, Miguel; Carrasco, Basilio; Retamales, Jorge B.; Caligari, Peter D. S.; García-González, Rolando

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dc.contributor.author	Quiroz, Karla
dc.contributor.author	Berríos- Muñoz, Miguel
dc.contributor.author	Carrasco, Basilio
dc.contributor.author	Retamales, Jorge B.
dc.contributor.author	Caligari, Peter D. S.
dc.contributor.author	García-González, Rolando
dc.date.accessioned	2017-09-11T13:05:51Z
dc.date.available	2017-09-11T13:05:51Z
dc.date.issued	2017
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/131
dc.description.abstract	Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propaga‑ tion, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable efort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propaga‑ tion technologies in the less studied species Fragaria chiloensis. Results: In this context, we studied the cultivation of meristems and establishment of a micropropagation proto‑ col for F. chiloensis. The addition of polyvinylpyrrolidone (PVP) improved the meristem regeneration efciency of F. chiloensis accessions. Similarly, the use of 6-benzylaminopurine (BAP) in the culture media increased the average rate of multiplication to 3–6 shoots per plant. In addition, the use of 6-benzylaminopurine (BAP), had low levels (near zero) of explant losses due to oxidation. However, plant height as well as number of leaves and roots were higher in media without growth regulators, with average values of 0.5 cm, 9 leaves and 4 roots per plant. Conclusions: For the frst time in Chilean strawberry, meristem culture demonstrated to be an efcient tool for elimi‑ nating virus from infected plants, giving the possibility to produce disease free propagation material. Also, the addi‑ tion of PVP into the basal MS medium improved the efciency of plant recovery from isolated meristems. Farmers can now access to high quality plant material produced by biotech tools which will improve their technological practices	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	Biological Research, 50, 20	es_CL
dc.subject	Fragaria chiloensis	es_CL
dc.subject	Meristem culture	es_CL
dc.subject	Plant growth regulators	es_CL
dc.subject	Virus elimination	es_CL
dc.subject	Plant morphogenesis	es_CL
dc.title	Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)	es_CL
dc.type	Article	es_CL
dc.ucm.facultad	Facultad de Ciencias Agrarias y Forestales	es_CL
dc.ucm.indexacion	Scopus	es_CL
dc.ucm.indexacion	Isi	es_CL
dc.ucm.indexacion	Scielo	es_CL
dc.ucm.doi	doi.org/10.1186/s40659-017-0125-8	es_CL