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dc.contributor.authorCordero, Cecilia
dc.contributor.authorCáceres-Ruz, Pablo
dc.contributor.authorGonzález-Silva, Gloria
dc.contributor.authorQuiroz, Karla
dc.contributor.authorBravo, Carmen
dc.contributor.authorRamírez, Ricardo
dc.contributor.authorCaligari, Peter D. S.
dc.contributor.authorCarrasco, Basilio
dc.contributor.authorGarcía-González, Rolando
dc.date.accessioned2017-11-30T19:24:05Z
dc.date.available2017-11-30T19:24:05Z
dc.date.issued2011
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/1514
dc.description.abstractTruffle (Tuber melanosporum Vitt.) culture is an agroforestry sector in Chile of increasing interest due to the high prices that truffles fetch in the national market and the recent evidence that its commercial production is possible in Chilean climatic and soil conditions. In this study, the efficiency of three methods of DNA extraction from a mix of 5 g of soil and roots from both nursery and field plants of Quercus ilex L. mycorrhized with T. melanosporum were evaluated, and a simple and reproducible protocol was established. Detection of T. melanosporum was performed by the technique of cleaved amplified polymorphic sequence (CAPS) from amplicons generated with the primers ADL1 (5´-GTAACGATAAAGGCCATCTATAGG-3´) and ADL3 (5´-CGTTTTTCCTGAACTCTTCATCAC-3`), where a restriction fragment of 160 bp specific for T. melanosporum was generated, which allows the discrimination of this species from the rest of the species belonging to the Tuber sp. genus. Direct detection of T. melanosporum in one step was also obtained by polymerase chain reaction (PCR) from total DNA isolated from mycorrhized roots and with the primers ITSML (5´-TGGCCATGTGTCAGATTTAGTA-3´) and ITSLNG (5´-TGATATGCTTAAGTTCAGCGGG-3´), generating a single amplicon of 440 bp. The molecular detection of T. melanosporum by the methods presented here will allow the rapid and accurate detection of mycorrhization of trees, both under nursery and field conditions. This technology will also provide more security to farmers by controlling the quality of the mycorrhized trees they will plant and also by following the mycorrhization status of established orchards.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceChilean Journal of Agricultural Research, 71(3), 488-494es_CL
dc.subjectTuber melanosporumes_CL
dc.subjectEctomycorrhizases_CL
dc.subjectMolecular markerses_CL
dc.titleMolecular tools for rapid and accurate detection of black truff (Tuber melanosporum Vitt.) in inoculated nursery plants and commercial plantations in Chilees_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias Agrarias y Forestaleses_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.indexacionScieloes_CL
dc.ucm.doidx.doi.org/10.4067/S0718-58392011000300022es_CL


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Atribución-NoComercial-SinDerivadas 3.0 Chile
Excepto si se señala otra cosa, la licencia de la publicación se describe como Atribución-NoComercial-SinDerivadas 3.0 Chile