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dc.contributor.authorMartínez, Irene
dc.contributor.authorZelada, Patricio
dc.contributor.authorGuevara, Felipe
dc.contributor.authorAndler-Osorio, Rodrigo
dc.contributor.authorUrtuvia, Viviana
dc.contributor.authorPacheco-Leyva, Ivette
dc.contributor.authorDíaz-Barrera, Álvaro
dc.date.accessioned2019-12-12T14:57:23Z
dc.date.available2019-12-12T14:57:23Z
dc.date.issued2019
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/2530
dc.description.abstractCoenzyme Q (CoQ) plays an important role as an electron transporter in the respiratory chain. It is formed from a benzoquinone ring and an isoprenoid chain of a specific length depending on the organism. We constructed an engineered Escherichia coli strain (menF) unable to produce demethylmenaquinone and menaquinone, compounds that compete for both chorismate, precursor of the benzoquinone ring, and the isoprenoid chain involved in CoQ biosynthesis. In addition, a mutant strain (entC) unable to produce enterobactin, high-affinity siderophore, synthesized from chorismate, and a double mutant (entC–menF) were constructed. The use of glucose or glycerol as carbon sources was also evaluated for the production of CoQ8 in these strains. The double mutant (entC–menF) showed 18% increase in CoQ8-specific content compared to the control strain; however, the single-mutant strains did not show statistically significant differences in CoQ8-specific content respect to the control, in glucose medium in bioreactor experiments. Glycerol was significantly superior compared to glucose for the production of CoQ8 in E. coli, where the CoQ8-specific content increased 126% and 53% in the control and double-mutant strain, respectively. The expression of genes related to CoQ8 biosynthesis is reported, where the entC–menF double-mutant strain showed a significant increase in the expression of CoQ8 biosynthesis-related genes when glycerol was used as sole carbon source. The control strain did not show gene expression difference between both carbon sources, indicating a possible regulation at a different level.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceBioprocess and Biosystems Engineering, 42(7), 1143-1149es_CL
dc.subjectCoenzyme Qes_CL
dc.subjectEscherichia colies_CL
dc.subjectCarbon sourcees_CL
dc.subjectMetabolic engineeringes_CL
dc.subjectGlyceroles_CL
dc.titleCoenzyme Q production by metabolic engineered Escherichia coli strains in defined mediumes_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias Agrarias y Forestaleses_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.urisibib2.ucm.cl:2048/login?url=https://link.springer.com/article/10.1007/s00449-019-02111-yes_CL
dc.ucm.doidoi.org/10.1007/s00449-019-02111-yes_CL


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