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Biotransformation of poly(cis-1,4-isoprene) in a multiphase enzymatic reactor for continuous extraction of oligo-isoprenoid molecules
| dc.contributor.author | Andler-Osorio, Rodrigo | |
| dc.contributor.author | Valdés, Cristian | |
| dc.contributor.author | Díaz-Barrera, Álvaro | |
| dc.contributor.author | Steinbüchel, A. | |
| dc.date.accessioned | 2020-10-28T12:01:00Z | |
| dc.date.available | 2020-10-28T12:01:00Z | |
| dc.date.issued | 2020 | |
| dc.identifier.uri | http://repositorio.ucm.cl/handle/ucm/3142 | |
| dc.description.abstract | Biotechnological processes for the partial degradation or transformation of poly(cis-1,4-isoprene) rubber have been investigated during recent decades with promising results. The use of the enzyme ‘latex clearing protein’ (Lcp) to transform the polymer into more hydrophilic oligo-isoprenoids results in modifications of the rubber structure and the synthesis of new material. In order to find an alternative process to recover the degradation products, a continuous extraction method using a biphasic system is described. The enzymatic activity of Lcp1VH2 was studied in the presence of ethyl acetate and pentane as extraction solvents. Oligo(cis-1,4-isoprene) molecular species were isolated from the organic phase and analyzed by Electrospray Ionization Mass Spectrometry. The enzymatic reaction process was evaluated in terms of the biotransformation yield of poly(cis-1,4-isoprene) rubber into the corresponding degradation products. Biotransformation yields of between 42–52 % were achieved depending on the enzymatic reactor design and the extraction solvent. The results also showed that the mass distribution of the oligo(cis-1,4-isoprene) depended on the organic solvent applied. A novel, simple and effective process is demonstrated for biotransformation of poly(cis-1,4-isoprene) rubber with high oligo-isoprenoid molecules recovery yields. | es_CL |
| dc.language.iso | en | es_CL |
| dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | * |
| dc.source | New Biotechnology, 58, 10-16 | es_CL |
| dc.subject | Latex clearing protein | es_CL |
| dc.subject | Multi-phase reactor | es_CL |
| dc.subject | Rubber biotransformation | es_CL |
| dc.subject | Oligo (cis-14-isoprene) | es_CL |
| dc.subject | Polymer degradation | es_CL |
| dc.title | Biotransformation of poly(cis-1,4-isoprene) in a multiphase enzymatic reactor for continuous extraction of oligo-isoprenoid molecules | es_CL |
| dc.type | Article | es_CL |
| dc.ucm.facultad | Facultad de Ciencias Agrarias y Forestales | es_CL |
| dc.ucm.indexacion | Scopus | es_CL |
| dc.ucm.indexacion | Isi | es_CL |
| dc.ucm.uri | sibib2.ucm.cl:2048/login?url=https://www.sciencedirect.com/science/article/abs/pii/S1871678420301217 | es_CL |
| dc.ucm.doi | doi.org/10.1016/j.nbt.2020.05.001 | es_CL |
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Excepto si se señala otra cosa, la licencia de la publicación se describe como Atribución-NoComercial-SinDerivadas 3.0 Chile