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Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions
| dc.contributor.author | Díaz-Barrera, Álvaro | |
| dc.contributor.author | Sánchez-Rosales, Francisco | |
| dc.contributor.author | Padilla-Córdova, Claudio | |
| dc.contributor.author | Andler-Osorio, Rodrigo | |
| dc.contributor.author | Peña, Carlos | |
| dc.date.accessioned | 2021-12-14T12:23:21Z | |
| dc.date.available | 2021-12-14T12:23:21Z | |
| dc.date.issued | 2021 | |
| dc.identifier.uri | http://repositorio.ucm.cl/handle/ucm/3576 | |
| dc.description.abstract | Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition. In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions. An increase in the maximum OTR (OTRmax) improved the alginate production, reaching 3.3 ± 0.2 g L−1. In the cultures conducted to an OTR of 10.4 mmol L−1 h−1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L−1 h−1 was constant throughout the cultivation. A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L−1 h−1. The OTR as a criterion to scale up alginate production allowed to replicate the concentration and the alginate production rate; however, it was not possible reproduce the MW and G/M ratio. Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g−1 h−1) the alginate MW was similar (approximately 365 kDa) in both scales. The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales. Overall, the results have shown that the alginate composition could be affected by cellular respiration, and from a technological perspective the evidences contribute to the design process based on oxygen consumption to produce alginates defined. | es_CL |
| dc.language.iso | en | es_CL |
| dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | * |
| dc.source | Bioprocess and Biosystems Engineering, 44, 1275-1287 | es_CL |
| dc.subject | Azotobacter vinelandii | es_CL |
| dc.subject | Alginate | es_CL |
| dc.subject | Molecular weight | es_CL |
| dc.subject | Oxygen transfer rate | es_CL |
| dc.title | Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions | es_CL |
| dc.type | Article | es_CL |
| dc.ucm.facultad | Facultad de Ciencias Agrarias y Forestales | es_CL |
| dc.ucm.indexacion | Scopus | es_CL |
| dc.ucm.indexacion | Isi | es_CL |
| dc.ucm.uri | link.springer.com/article/10.1007%2Fs00449-021-02532-8 | es_CL |
| dc.ucm.doi | doi.org/10.1007/s00449-021-02532-8 | es_CL |
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