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Deletion of hippocampal Glucocorticoid receptors unveils sex-biased microRNA expression and neuronal morphology alterations in mice

dc.contributor.authorTejos-Bravo, Macarena
dc.contributor.authorOakley, Robert H.
dc.contributor.authorWhirledge, Shannon D.
dc.contributor.authorCorrales, Wladimir
dc.contributor.authorSilva, Juan P.
dc.contributor.authorGarcía-Rojo, Gonzalo
dc.contributor.authorToledo, Jorge
dc.contributor.authorSanchez, Wendy
dc.contributor.authorRomán-Albasini, Luciano
dc.contributor.authorAliaga-Rojas, Esteban
dc.contributor.authorAguayo, Felipe
dc.contributor.authorOlave, Felipe A.
dc.contributor.authorMarcaja-Coutinho, Vinicius
dc.contributor.authorCidlowski, John A.
dc.contributor.authorFiedler, Jenny L.
dc.date.accessioned2021-12-15T13:46:40Z
dc.date.available2021-12-15T13:46:40Z
dc.date.issued2021
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/3620
dc.description.abstractSex differences in the brain have prompted many researchers to investigate the underlying molecular actors, such as the glucocorticoid receptor (GR). This nuclear receptor controls gene expression, including microRNAs (miRNAs), in non-neuronal cells. Here, we investigated sex-biased effects of GR on hippocampal miRNA expression and neuronal morphology by generating a neuron-specific GR knockout mouse (Emx1-Nr3c1−/−). The levels of 578 mature miRNAs were assessed using NanoString technology and, in contrast to males, female Emx1-Nr3c1−/− mice showed a substantially higher number of differentially expressed miRNAs, confirming a sex-biased effect of GR ablation. Based on bioinformatic analyses we identified several transcription factors potentially involved in miRNA regulation. Functional enrichment analyses of the miRNA-mRNA interactions revealed pathways related to neuronal arborization and both spine morphology and density in both sexes. Two recognized regulators of dendritic morphology, CAMKII-α and GSK-3β, increased their protein levels by GR ablation in female mice hippocampus, without changes in males. Additionally, sex-specific effects of GR deletion were observed on CA1 neuronal arborization and dendritic spine features. For instance, a reduced density of mushroom spines in apical dendrites was evidenced only in females, while a decreased length in basal dendrites was noted only in males. However, length and arborization of apical dendrites were reduced by GR ablation irrespective of the sex. Overall, our study provides new insights into the sex-biased GR actions, especially in terms of miRNAs expression and neuronal morphology in the hippocampus.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceNeurobiology of Stress, 14, 100306es_CL
dc.subjectGlucocorticoid receptores_CL
dc.subjectHippocampuses_CL
dc.subjectNeuroplasticityes_CL
dc.subjectDendriteses_CL
dc.subjectDendritic spineses_CL
dc.subjectSexes_CL
dc.titleDeletion of hippocampal Glucocorticoid receptors unveils sex-biased microRNA expression and neuronal morphology alterations in micees_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias de la Saludes_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.uriwww.sciencedirect.com/science/article/pii/S235228952100014X?via%3Dihubes_CL
dc.ucm.doidoi.org/10.1016/j.ynstr.2021.100306es_CL


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Atribución-NoComercial-SinDerivadas 3.0 Chile
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