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TRPM8 channel promotes the osteogenic differentiation in human bone marrow mesenchymal stem cells

dc.contributor.authorHenao, Juan C.
dc.contributor.authorGrismaldo, Adriana
dc.contributor.authorBarreto, Alfonso
dc.contributor.authorRodríguez-Pardo, Viviana M.
dc.contributor.authorMejía-Cruz, Claudia C.
dc.contributor.authorLeal-Garcia, Efrain
dc.contributor.authorPérez-Núñez, Rafael
dc.contributor.authorRojas, Patricio
dc.contributor.authorLatorre, Ramón
dc.contributor.authorCarvacho-Contreras, Ingrid
dc.contributor.authorTorres, Yolima P.
dc.date.accessioned2022-07-06T15:23:28Z
dc.date.available2022-07-06T15:23:28Z
dc.date.issued2021
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/3860
dc.description.abstractVarious families of ion channels have been characterized in mesenchymal stem cells (MSCs), including some members of transient receptor potential (TRP) channels family. TRP channels are involved in critical cellular processes as differentiation and cell proliferation. Here, we analyzed the expression of TRPM8 channel in human bone marrow MSCs (hBM-MSCs), and its relation with osteogenic differentiation. Patch-clamp recordings showed that hBM-MSCs expressed outwardly rectifying currents which were increased by exposure to 500 μM menthol and were partially inhibited by 10 μM of BCTC, a TRPM8 channels antagonist. Additionally, we have found the expression of TRPM8 by RT-PCR and western blot. We also explored the TRPM8 localization in hBM-MSCs by immunofluorescence using confocal microscopy. Remarkably, hBM-MSCs treatment with 100 μM of menthol or 10 μM of icilin, TRPM8 agonists, increases osteogenic differentiation. Conversely, 20 μM of BCTC, induced a decrease of osteogenic differentiation. These results suggest that TRPM8 channels are functionally active in hBM-MSCs and have a role in cell differentiation.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceFrontiers in Cell and Developmental Biology, 9es_CL
dc.subjectTRP channelses_CL
dc.subjectBone marrow mesenchymal stem cellses_CL
dc.subjectOsteogenic differentiationes_CL
dc.subjectMesenchymal stem celles_CL
dc.subjectTRPM8 channeles_CL
dc.titleTRPM8 channel promotes the osteogenic differentiation in human bone marrow mesenchymal stem cellses_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias Básicases_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.uriwww.frontiersin.org/articles/10.3389/fcell.2021.592946/full#:~:text=TRPM8%20Channel%20Promotes%20the%20Osteogenic%20Differentiation%20in%20Human%20Bone%20Marrow%20Mesenchymal%20Stem%20Cells,-Juan%20C.&text=Various%20families%20of%20ion%20channels,potential%20(TRP)%20channels%20family.es_CL
dc.ucm.doidoi.org/10.3389/fcell.2021.592946es_CL


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Atribución-NoComercial-SinDerivadas 3.0 Chile
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