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Sex-dependent changes of miRNA levels in the hippocampus of adrenalectomized rats following acute corticosterone administration

dc.contributor.authorCorrales, Wladimir
dc.contributor.authorSilva, Juan P.
dc.contributor.authorParra, Claudio S.
dc.contributor.authorOlave, Felipe A.
dc.contributor.authorAguayo, Felipe
dc.contributor.authorRomán-Albasini, Luciano
dc.contributor.authorAliaga-Rojas, Esteban
dc.contributor.authorVenegas-Zamora, Leslye
dc.contributor.authorAvalos, Ana M.
dc.contributor.authorRojas, P.S.
dc.contributor.authorMarcaja-Coutinho, Vinicius
dc.contributor.authorOakley, Robert H.
dc.contributor.authorCidlowski, John A.
dc.contributor.authorFiedler, Jenny L.
dc.date.accessioned2022-08-02T15:30:21Z
dc.date.available2022-08-02T15:30:21Z
dc.date.issued2021
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/3962
dc.description.abstractWe explored sex-biased effects of the primary stress glucocorticoid hormone corticosterone on the miRNA expression profile in the rat hippocampus. Adult adrenalectomized (ADX) female and male rats received a single corticosterone (10 mg/kg) or vehicle injection, and after 6 h, hippocampi were collected for miRNA, mRNA, and Western blot analyses. miRNA profiling microarrays showed a basal sex-biased miRNA profile in ADX rat hippocampi. Additionally, acute corticosterone administration triggered a sex-biased differential expression of miRNAs derived from genes located in several chromosomes and clusters on the X and 6 chromosomes. Putative promoter analysis unveiled that most corticosterone-responsive miRNA genes contained motifs for either direct or indirect glucocorticoid actions in both sexes. The evaluation of transcription factors indicated that almost 50% of miRNA genes sensitive to corticosterone in both sexes was under glucocorticoid receptor regulation. Transcription factor-miRNA regulatory network analyses identified several transcription factors that regulate, activate, or repress miRNA expression. Validated target mRNA analysis of corticosterone-responsive miRNAs showed a more complex miRNA–mRNA interaction network in males compared to females. Enrichment analysis revealed that several hippocampal-relevant pathways were affected in both sexes, such as neurogenesis and neurotrophin signaling. The evaluation of selected miRNA targets from these pathways displayed a strong sex difference in the hippocampus of ADX-vehicle rats. Corticosterone treatment did not change the levels of the miRNA targets and their corresponding tested proteins. Our data indicate that corticosterone exerts a sex-biased effect on hippocampal miRNA expression, which may engage in sculpting the basal sex differences observed at higher levels of hippocampal functioning.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceACS Chemical Neuroscience, 12(16), 2981-3001es_CL
dc.subjectAdrenalectomyes_CL
dc.subjectCorticosteronees_CL
dc.subjectGlucocorticoid receptorses_CL
dc.subjectmiRNAses_CL
dc.subjectHippocampuses_CL
dc.subjectSex biases_CL
dc.titleSex-dependent changes of miRNA levels in the hippocampus of adrenalectomized rats following acute corticosterone administrationes_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias de la Saludes_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.uripubs.acs.org/doi/10.1021/acschemneuro.0c00762es_CL
dc.ucm.doidoi.org/10.1021/acschemneuro.0c00762es_CL


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Atribución-NoComercial-SinDerivadas 3.0 Chile
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