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Deletion of TRPV3 and CaV3.2 T-type channels in mice undermines fertility and Ca2+ homeostasis in oocytes and eggs

dc.contributor.authorMehregan, Aujan
dc.contributor.authorArdestani, Goli
dc.contributor.authorAkizawa, Hiroki
dc.contributor.authorCarvacho-Contreras, Ingrid
dc.contributor.authorFissore, Rafael A.
dc.date.accessioned2022-08-19T13:09:14Z
dc.date.available2022-08-19T13:09:14Z
dc.date.issued2021
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/3996
dc.description.abstractCa2+ influx during oocyte maturation and after sperm entry is necessary to fill the internal Ca2+ stores and for complete egg activation. We knocked out the transient receptor potential vanilloid member 3 (TRPV3) and the T-type channel, CaV3.2, to determine their necessity for maintaining these functions in mammalian oocytes/eggs. Double-knockout (dKO) females were subfertile, their oocytes and eggs showed reduced internal Ca2+ stores, and, following sperm entry or Plcz (also known as Plcz1) cRNA injection, fewer dKO eggs displayed Ca2+ responses compared to wild-type eggs, which were also of lower frequency. These parameters were rescued and/or enhanced by removing extracellular Mg2+, suggesting that the residual Ca2+ influx could be mediated by the TRPM7 channel, consistent with the termination of divalent-cation oscillations in dKO eggs by a TRPM7 inhibitor. In total, we demonstrated that TRPV3 and CaV3.2 mediate the complete filling of the Ca2+ stores in mouse oocytes and eggs. We also showed that they are required for initiating and maintaining regularly spaced-out oscillations, suggesting that Ca2+ influx through PM ion channels dictates the periodicity and persistence of Ca2+ oscillations during mammalian fertilization.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceJournal of Cell Science, 134(13), jcs257956es_CL
dc.subjectOocytees_CL
dc.subjectFertilizationes_CL
dc.subjectCa2+es_CL
dc.subjectSignalinges_CL
dc.subjectTRP channeles_CL
dc.titleDeletion of TRPV3 and CaV3.2 T-type channels in mice undermines fertility and Ca2+ homeostasis in oocytes and eggses_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias Básicases_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.urijournals.biologists.com/jcs/article/134/13/jcs257956/270886/Deletion-of-TRPV3-and-CaV3-2-T-type-channels-ines_CL
dc.ucm.doidoi.org/10.1242/jcs.257956es_CL


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Atribución-NoComercial-SinDerivadas 3.0 Chile
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