Validation of reference genes in a population of blueberry (vaccinium corymbosum) plants regenerated in colchicine

D'Afonseca, Vívian; Hernández, Ricardo; Gómez, Aleydis; Arencibia-Rodríguez, Ariel; Valenzuela-Valenzuela, Francisca

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dc.contributor.author	D'Afonseca, Vívian
dc.contributor.author	Hernández, Ricardo
dc.contributor.author	Gómez, Aleydis
dc.contributor.author	Arencibia-Rodríguez, Ariel
dc.contributor.author	Valenzuela-Valenzuela, Francisca
dc.date.accessioned	2022-12-06T20:26:32Z
dc.date.available	2022-12-06T20:26:32Z
dc.date.issued	2022
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/4193
dc.description.abstract	For the first time we report the validation of reference genes in plants from a population of blueberry (Vaccinium corymbosum) clones cultured in vitro on a colchicine-supplemented medium. Nodal segment explants of the cultivar Duke were regenerated by organogenesis under different periods of colchicine 1 mg/L exposure (1, 2, 3, 5, 30 days). The clones selected for the study showed variability for phenotypic traits after 2 years of adaptation to field conditions, compared to plants of the donor genotype that were regenerated on a medium without colchicine. Vaccinium myrtillus (GAPDH) and Vaccinium macrocarpon (ATP1, NADH, RPOB and COX2) were used as reference genomes for primer design. The results show that colchicine treatments can cause genomic changes in blueberry plants. At the molecular level, exposure of plants to colchicine in early periods could promote an increase in gene expression of specific genes such as ATP1, COX2, GAPDH, MATK, NADH and RPOB. However, prolonged exposure (30 days) could decrease gene expression of the genes studied. For qPCR assays, the primers designed for ATP1, COX2, GAPDH and MATK genes showed high efficiency. In addition, the GAPDH, ATP1, NADH and COX2 genes showed high stability and could be recommended as potential reference genes for gene expression assays in Vaccinium.	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	Plants, 11(19), 2645	es_CL
dc.subject	Reference genes	es_CL
dc.subject	Gene expression	es_CL
dc.subject	Colchicine	es_CL
dc.subject	RT-qPCR	es_CL
dc.subject	Vaccinium corymbosum	es_CL
dc.title	Validation of reference genes in a population of blueberry (vaccinium corymbosum) plants regenerated in colchicine	es_CL
dc.type	Article	es_CL
dc.ucm.facultad	Facultad de Ciencias Agrarias y Forestales	es_CL
dc.ucm.indexacion	Scopus	es_CL
dc.ucm.uri	mdpi.com/2223-7747/11/19/2645	es_CL
dc.ucm.doi	doi.org/10.3390/plants11192645	es_CL