In order to gain a molecular understanding of why quercetin characteristics are altered by glucosylation, DFT calculations and MD simulations were utilized. According to DFT findings, glucosylation of quercetin altered its structural stability, molecular polarity, molecular polarizability, and Gibbs free energy of transfer, which gives a molecular explanation for the observed alterations in the aqueous solubility and lipophilicity of derivatives. The outcomes of MD simulations also demonstrated how the various hydroxyl groups of quercetin interact with solvent molecules and how glucosylation can have an impact on that. Additionally, it was shown that glucosylation often increased the number of hydrogen bonds and the nonbonded interaction energies between the molecules of the solvent and the solute. The solubility behavior of glucosylated derivatives in water and alcoholic solvents can be reasonably interpreted molecularly using these results, which could suggest approaches for improving the features of these kinds of bioactive compounds.