A rapid and simple protocol for the isolation of bacteriophages from coastal organisms

Echeverría-Vega, Alex; Morales-Vicencio, Pablo; Saez-Saavedra, Camila; Gordillo-Fuenzalida, Felipe; Araya, Rubén

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dc.contributor.author	Echeverría-Vega, Alex
dc.contributor.author	Morales-Vicencio, Pablo
dc.contributor.author	Saez-Saavedra, Camila
dc.contributor.author	Gordillo-Fuenzalida, Felipe
dc.contributor.author	Araya, Rubén
dc.date.accessioned	2023-03-22T17:40:59Z
dc.date.available	2023-03-22T17:40:59Z
dc.date.issued	2019
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/4551
dc.description.abstract	This work details a protocol for recovering bacteriophages from intertidal sessile mussels and testing their lytic activity against pathogenic bacteria. Although bacteriophages were highly abundant in coastal filter-feeding organisms, they were not detectable in the surrounding water column. This difference reflects the high filtering rate of the mussels, which capture and concentrate high amounts of bacteria, generating an ideal environment for bacteriophages. We validated the protocol providing a mean concentration of 4E + 04 PFU mL-1 lytic bacteriophages for the fish-pathogen bacterium Vibrio ordalii. We suggest that this method has particular utility for the recovery of bacteriophages for use as natural antimicrobial agents in aquaculture.	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	MethodsX, 6, 2614-2619	es_CL
dc.subject	Vibrio bacteriophage	es_CL
dc.subject	Phage prospection	es_CL
dc.subject	Phage isolation	es_CL
dc.title	A rapid and simple protocol for the isolation of bacteriophages from coastal organisms	es_CL
dc.type	Article	es_CL
dc.ucm.indexacion	Scopus	es_CL
dc.ucm.uri	methods-x.com/article/S2215-0161(19)30305-X/fulltext	es_CL
dc.ucm.doi	doi.org/10.1016/j.mex.2019.11.003	es_CL