Mostrar el registro sencillo de la publicación
Effect of strand register in the stability and reactivity of crystals from peptides forming amyloid fibrils
| dc.contributor.author | Muñoz-Gutiérrez, Camila | |
| dc.contributor.author | Adasme-Carreño, Francisco | |
| dc.contributor.author | Alzate-Morales, Jans | |
| dc.contributor.author | Ireta, Joel | |
| dc.date.accessioned | 2025-03-12T13:45:34Z | |
| dc.date.available | 2025-03-12T13:45:34Z | |
| dc.date.issued | 2023 | |
| dc.identifier.uri | http://repositorio.ucm.cl/handle/ucm/5848 | |
| dc.description.abstract | Amyloids are cytotoxic protein aggregates that deposit in human tissues, leading to several health disorders. Their aggregates can also exhibit catalytic properties, and they have been used as candidates for the development of functional biomaterials. Despite being polymorphic, amyloids often assemble as cross-β fibrils formed by in-register β sheet layers. Recent studies of some amyloidogenic protein segments revealed that they crystallize as antiparallel out-of-register β sheets. Such arrangement has been proposed to be responsible for the cytotoxicity in amyloid diseases, however, there is still no consensus on the molecular mechanism. Interestingly, two amyloidogenic peptide segments, NFGAILS and FGAILSS, arrange into out-of-register and in-register β sheets, respectively, even though they solely differ by one aminoacid residue at both termini. In this work, we used density functional theory (DFT) to address how the strand register contributes into the packing and molecular properties of the NFGAILS and FGAILSS crystals. Our results show that the out-of-register structure is substantially more stable, at 0 K, than the in-register one due to stronger inter-strand contacts. Based on an analysis of the electrostatic potential of the crystal slabs, it is suggested that the out-of-register may potentially interact with negatively charged groups, like those found in cell membranes. Moreover, calculated reactivity descriptors indicate a similar outcome, where only the out-of-register peptide exhibits intrinsic reactive surface sites at the exposed amine and carboxylic groups. It is therefore suggested that the out-of-register arrangement may indeed be crucial for amyloid cytotoxicity. The findings presented here could help to further our understanding of amyloid aggregation, function, and toxicity. | es_CL |
| dc.language.iso | en | es_CL |
| dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | * |
| dc.source | Physical Chemistry Chemical Physics, 25(35), 23885-23893 | es_CL |
| dc.title | Effect of strand register in the stability and reactivity of crystals from peptides forming amyloid fibrils | es_CL |
| dc.type | Article | es_CL |
| dc.ucm.facultad | Facultad de Medicina | es_CL |
| dc.ucm.indexacion | Scopus | es_CL |
| dc.ucm.indexacion | Isi | es_CL |
| dc.ucm.uri | pubs.rsc.org/en/content/articlelanding/2023/CP/D3CP01762A | es_CL |
| dc.ucm.doi | doi.org/10.1039/D3CP01762A | es_CL |
Ficheros en la publicación
| Ficheros | Tamaño | Formato | Ver |
|---|---|---|---|
|
No hay ficheros asociados a esta publicación. |
|||
Esta publicación aparece en la(s) siguiente(s) colección(ones)
Excepto si se señala otra cosa, la licencia de la publicación se describe como Atribución-NoComercial-SinDerivadas 3.0 Chile