Ver publicación 

Mostrar el registro sencillo de la publicación

Dual effect of serotonin on the dendritic growth of cultured hippocampal neurons: Involvement of 5-HT1A and 5-HT7 receptors

dc.contributor.authorAliaga-Rojas, Esteban
dc.contributor.authorRojas, P.S.
dc.contributor.authorAguayo, Felipe I.
dc.contributor.authorNeira, D.
dc.contributor.authorMuñoz, J.P.
dc.contributor.authorTejos-Bravo, Macarena
dc.contributor.authorParra, Claudio S.
dc.contributor.authorFiedler, Jenny L.
dc.date.accessioned2017-10-30T15:20:49Z
dc.date.available2017-10-30T15:20:49Z
dc.date.issued2017
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/944
dc.description.abstractSerotonin acts through its receptors (5-HTRs) to shape brain networks during development and modulates essential functions in mature brain. The 5-HT1AR is mainly located at soma of hippocampal neurons early during brain development and its expression gradually shifts to dendrites during postnatal development. The 5-HT7R expressed early during hippocampus development, shows a progressive reduction in its expression postnatally. Considering these changes during development, we evaluated in cultured hippocampal neurons whether the 5- HT1AR and 5-HT7R change their expression, modulate dendritic growth, and activate signaling pathways such as ERK1/2, AKT/GSK3β and LIMK/cofilin, which may sustain dendrite outgrowth by controlling cytoskeleton dynamics. We show that mRNA levels of both receptors increase between 2 and 7 DIV; however only protein levels of 5- HT7R increase significantly at 7 DIV. The 5-HT1AR is preferentially distributed in the soma, while 5-HT7R displays a somato-dendritic localization at 7 DIV. Through stimulation with 5-HT at 7 DIV during 24 h and using specific antagonists, we determined that 5-HT1AR decreases the number of primary and secondary dendrites and restricts the growth of primary dendrites. The activation of 5-HT1AR and 5-HT7R promotes the growth of short secondary dendrites and triggers ERK1/2 and AKT phosphorylation through MEK and PI3K activation respectively; without changes in the phosphorylation of LIMK and cofilin. We conclude that 5-HT1AR restricts dendritogenesis and outgrowth of primary dendrites, but that both 5-HT1AR and 5-HT7R promote secondary dendrite outgrowth. These data support the role of 5-HT in neuronal outgrowth during development and provide insight into cellular basis of neurodevelopmental disorders.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceMolecular and Cellular Neuroscience, 85, 148–161es_CL
dc.subjectSerotonines_CL
dc.subject5-HT1ARes_CL
dc.subject5-HT7Res_CL
dc.subjectOutgrowthes_CL
dc.subjectDendritees_CL
dc.subjectHippocampal neuronses_CL
dc.subjectERK1/2es_CL
dc.subjectAKTes_CL
dc.subjectGSK3βes_CL
dc.subjectLIMKes_CL
dc.subjectCofilines_CL
dc.titleDual effect of serotonin on the dendritic growth of cultured hippocampal neurons: Involvement of 5-HT1A and 5-HT7 receptorses_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias de la Saludes_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.urisibib2.ucm.cl:2048/login?url=https://www.sciencedirect.com/science/article/pii/S104474311730180X?via%3Dihubes_CL
dc.ucm.doidoi.org/10.1016/j.mcn.2017.09.009es_CL


Ficheros en la publicación

FicherosTamañoFormatoVer

No hay ficheros asociados a esta publicación.

Esta publicación aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo de la publicación

Atribución-NoComercial-SinDerivadas 3.0 Chile
Excepto si se señala otra cosa, la licencia de la publicación se describe como Atribución-NoComercial-SinDerivadas 3.0 Chile