Ver publicación 

Mostrar el registro sencillo de la publicación

Zona pellucida removal modifies the expression and release of specific microRNAs in domestic cat blastocysts

dc.contributor.authorVeraguas-Dávila, Daniel
dc.contributor.authorCaamaño, Diego
dc.contributor.authorSaéz-Ruiz, Darling
dc.contributor.authorVásquez, Yazmín
dc.contributor.authorSaravia, Fernando
dc.contributor.authorOvidio Castro, Fidel
dc.contributor.authorRodríguez-Alvarez, Lleretny
dc.date.accessioned2025-03-17T18:08:52Z
dc.date.available2025-03-17T18:08:52Z
dc.date.issued2023
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/5850
dc.description.abstractThe in vitro culture of domestic cat embryos without the zona pellucida affects their implantation capacity. MicroRNAs (miRNAs) have an important role in embryo–maternal communication and implantation. The objective of this study was to evaluate the expression of specific miRNAs in domestic cat blastocysts cultured without the zona pellucida. Two experimental groups were done: (1) domestic cat embryos cultured with the zona pellucida (zona intact control group, ZI); and (2) cultured without the zona pellucida (zona free group, ZF). The cleavage, morula and blastocyst rates were evaluated. The blastocysts and their spent medium were used for miRNA expression analysis using RT-qPCR (miR-21, miR-24, mi25, miR-29, miR-96, miR-98, miR-103, miR-191, miR-196, miR-199, miR-130, miR-155 and miR-302). The pre-mature microRNAs (pre-miRNAs) and miRNAs were evaluated in the blastocysts and only miRNAs were evaluated in the spent medium. No differences were observed in the cleavage, morula and blastocyst rates between the ZF and ZI groups (P > 0.05). For miRNAs analysis, miR-103 and miR-191 had the most stable expression and were selected as internal controls. ZF blastocysts had a higher expression of miR-21, miR-25, miR-29 and miR-199 and a lower expression of miR-96 than their ZI counterparts (P < 0.05). Furthermore, higher levels of miR-21, miR-25 and miR-98 were detected in the spent medium of ZF blastocysts (P < 0.05). In conclusion, in vitro culture of domestic cat embryos without the zona pellucida modifies the expression of miR-21, miR-25, miR-29, miR-199 and miR-96 at the blastocyst stage and the release of miR-21, miR-25 and miR-98.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceZygote, 31(6), 544 - 556es_CL
dc.subjectFelid embryoses_CL
dc.subjectGene expressiones_CL
dc.subjectIn vitro fertilizationes_CL
dc.subjectMicroRNAses_CL
dc.titleZona pellucida removal modifies the expression and release of specific microRNAs in domestic cat blastocystses_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias Agrarias y Forestaleses_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.uricambridge.org/core/journals/zygote/article/abs/zona-pellucida-removal-modifies-the-expression-and-release-of-specific-micrornas-in-domestic-cat-blastocysts/E53E675EEA84F0CE424338A2CEA8D3E8es_CL
dc.ucm.doidoi.org/10.1017/S0967199423000436es_CL


Ficheros en la publicación

FicherosTamañoFormatoVer

No hay ficheros asociados a esta publicación.

Esta publicación aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo de la publicación

Atribución-NoComercial-SinDerivadas 3.0 Chile
Excepto si se señala otra cosa, la licencia de la publicación se describe como Atribución-NoComercial-SinDerivadas 3.0 Chile