Understanding brewing trait inheritance in de novo Lager yeast hybrids

Zavaleta, Vasni; Pérez-Través, Laura; Saona, Luis A.; Villarroel, Carlos A.; Querol, Amparo; Cubillos, Francisco A.

Mostrar el registro sencillo de la publicación

dc.contributor.author	Zavaleta, Vasni
dc.contributor.author	Pérez-Través, Laura
dc.contributor.author	Saona, Luis A.
dc.contributor.author	Villarroel, Carlos A.
dc.contributor.author	Querol, Amparo
dc.contributor.author	Cubillos, Francisco A.
dc.date.accessioned	2025-04-02T14:48:56Z
dc.date.available	2025-04-02T14:48:56Z
dc.date.issued	2024
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/5905
dc.description.abstract	Hybridization between Saccharomyces cerevisiae and Saccharomyces eubayanus resulted in the emergence of S. pastorianus, a crucial yeast for lager fermentation. However, our understanding of hybridization success and hybrid vigor between these two species remains limited due to the scarcity of S. eubayanus parental strains. Here, we explore hybridization success and the impact of hybridization on fermentation performance and volatile compound profiles in newly formed lager hybrids. By selecting parental candidates spanning a diverse array of lineages from both species, we reveal that the Beer and PB-2 lineages exhibit high rates of hybridization success in S. cerevisiae and S. eubayanus, respectively. Polyploid hybrids were generated through a spontaneous diploid hybridization technique (rare-mating), revealing a prevalence of triploids and diploids over tetraploids. Despite the absence of heterosis in fermentative capacity, hybrids displayed phenotypic variability, notably influenced by maltotriose consumption. Interestingly, ploidy levels did not significantly correlate with fermentative capacity, although triploids exhibited greater phenotypic variability. The S. cerevisiae parental lineages primarily influenced volatile compound profiles, with significant differences in aroma production. Interestingly, hybrids emerging from the Beer S. cerevisiae parental lineages exhibited a volatile compound profile resembling the corresponding S. eubayanus parent. This pattern may result from the dominant inheritance of the S. eubayanus aroma profile, as suggested by the over-expression of genes related to alcohol metabolism and acetate synthesis in hybrids including the Beer S. cerevisiae lineage. Our findings suggest complex interactions between parental lineages and hybridization outcomes, highlighting the potential for creating yeasts with distinct brewing traits through hybridization strategies.	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	mSystems, 9(12), e00762	es_CL
dc.subject	Yeast	es_CL
dc.subject	Beer	es_CL
dc.subject	Volatile compounds	es_CL
dc.subject	Lager	es_CL
dc.subject	Hybridization	es_CL
dc.subject	RNA-seq	es_CL
dc.title	Understanding brewing trait inheritance in de novo Lager yeast hybrids	es_CL
dc.type	Article	es_CL
dc.ucm.facultad	Facultad de Ciencias de la Ingeniería	es_CL
dc.ucm.indexacion	Scopus	es_CL
dc.ucm.indexacion	Isi	es_CL
dc.ucm.uri	journals.asm.org/doi/10.1128/msystems.00762-24	es_CL
dc.ucm.doi	doi.org/10.1128/msystems.00762-24	es_CL